Buffaloes share 44.1% of milk production in Maharashtra. Jafarabadi breed is highly rendered breed in western Maharashtra.(1) Lactation period in buffalo ranges from 295 to 300 days. Genetic and non-genetic factors affect lactation efficiency in buffalo.(2) National action plan of dairy development vision 2022 aims increase milk productivity throughout country. But we are facing challenges like low productivity of bovine and lack of preventive action against adulteration of milk and use of synthetic steroids as galactagogue i.e., oxytocin. It causes various deleterious effects like decrease fertility of cattle, excessive hemorrhage. The aim of this study was to observe effectiveness of Urtica urens 6C in increasing milk quantity in lactating Jafarabadi Buffaloes For 6 months from the same stable. Using cluster sampling technique, a sample of 30 healthy lactating, Jafarabadi buffaloes who had not yet received oxytocin injections were studied for 6 months. Pilot study for 15 days capturing the yield before administration of the medicine. For a total of six months, 15-day admission cycles of the medication and a placebo were repeated. After analyzing all data in SPSS, the results indicate that Urtica urens 6C is effective in boosting lactation efficiency in lactating Jafarabadi buffaloes